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p21 Cip1 antibody [GT8611]

KO/KD Orthogonal
Anti-p21 Cip1 antibody [GT8611] used in Flow cytometry (FACS). GTX629627
Anti-p21 Cip1 antibody [GT8611] used in Western Blot (WB). GTX629627
Anti-p21 Cip1 antibody [GT8611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629627
Anti-p21 Cip1 antibody [GT8611] used in Western Blot (WB). GTX629627

Cat. No. GTX629627

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT8611

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all p21 Cip1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HCT116 (30 μM Cisplatin treatment for 24 hr) , HeLa

Predict Reactivity

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888123

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p21 Cip1 antibody [GT8611] used in Flow cytometry (FACS). GTX629627

GTX629627 FACS Image

p21 Cip1 antibody [GT8611] (GTX629627) detects p21 Cip1 by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: p21 Cip1 antibody [GT8611] (GTX629627) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-p21 Cip1 antibody [GT8611] used in Western Blot (WB). GTX629627

GTX629627 WB Image

Orthogonal

p21 Cip1 antibody [GT8611] detects p21 Cip1 protein by Western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated)
B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24 hr)
15 % SDS-PAGE
p21 Cip1 antibody [GT8611] (GTX629627) dilution: 1:1000

Anti-p21 Cip1 antibody [GT8611] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629627

GTX629627 ICC/IF Image

p21 Cip1 antibody [GT8611] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells treated with 30μM cisplatin for 24hr and were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-p21 Cip1 antibody [GT8611] used in Western Blot (WB). GTX629627

GTX629627 WB Image

KO/KD

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT8611] (GTX629627) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Liao YC et al. Mol Ther Nucleic Acids 2023; 33 : 351-366 Tumor targeting and therapeutic assessments of RNA nanoparticles carrying α9-nAChR aptamer and anti-miR-21 in triple-negative breast cancers.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations