Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| Assay dependent |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. It is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors.
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human recombinant p21 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
CAP20 , CDKN1 , CDKN1A , CIP1 , MDA6 , P21 , SDI1 , WAF1 , cyclin dependent kinase inhibitor 1A , p21CIP1 , p21 , p21 Cip1
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]
Database
Research Area
![Anti-p21 Cip1 antibody [HJ21] used in IHC (Paraffin sections) (IHC-P). GTX34925](/upload/website/prouct_img/small/GTX34925/GTX34925_20200115_IHC-P_57_w_23060801_869.webp "Anti-p21 Cip1 antibody [HJ21] used in IHC (Paraffin sections) (IHC-P). GTX34925")
![Anti-p21 Cip1 antibody [HJ21] used in IHC (Paraffin sections) (IHC-P). GTX34925](/upload/website/prouct_img/normal/GTX34925/GTX34925_20200115_IHC-P_57_w_23060801_869.webp "Anti-p21 Cip1 antibody [HJ21] used in IHC (Paraffin sections) (IHC-P). GTX34925")