p21 Cip1 antibody [SPM306]

Cat. No. GTX34923

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM306
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

See all p21 Cip1 products

Datasheet
Document

View product citations for antibody GTX34923 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	2-4μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Product Note

This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant p21 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAP20 , CDKN1 , CDKN1A , CIP1 , MDA6 , P21 , SDI1 , WAF1 , cyclin dependent kinase inhibitor 1A , p21CIP1 , p21 , p21 Cip1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Gene ID: 1026 CDKN1A
UniProt: P38936 CDKN1A

Research Area

DATA IMAGES

GTX34923 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34923 p21 Cip1 antibody [SPM306].

GTX34923 WB Image

WB analysis of HeLa cell lysate using GTX34923 p21 Cip1 antibody [SPM306].

REFERENCE

There are currently no references for p21 Cip1 antibody [SPM306] (GTX34923). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for p21 Cip1 antibody [SPM306] (GTX34923). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty