GeneTex
United States (US)

p21 Cip1 antibody [WA-1]

p21-antibody-WA-1__GTX27903-2_18121411_897.gif
p21-antibody-WA-1__GTX27903-1_18121411_386.gif

Cat No. GTX27903

Host Mouse
Clonality Monoclonal
Clone Name WA-1
Isotype IgG1
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Monkey
APPLICATION

Application Note

ELISA: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

18 kDa. ( Note )

Specificity/Sensitivity

p21 protein [wild-type p53-activated fragment 1 (WAF-1) or Cdk-interacting protein (CIP1)].
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length native protein (purified) (Human).

Purification

Purified by precipitation methods

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase Inhibitor 1A,Cap20,Cdkn1,Cip1,Mda-6,P21,Sdi1,Waf1,P21Cip1,Cdkn1A

Cellular Localization

Nuclear

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Database

Research Area

DATA IMAGES
p21-antibody-WA-1__GTX27903-2_18121411_897.gif

GTX27903 IHC-P Image

Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1) (GTX27903).

p21-antibody-WA-1__GTX27903-1_18121411_386.gif

GTX27903 ICC/IF Image

Immunofluorescence staining of p21 in human primary fibroblasts using anti-p21 (WA-1 (GTX27903); green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).