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p21 Cip1 antibody

Orthogonal
Anti-p21 Cip1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100444
Anti-p21 Cip1 antibody used in Western Blot (WB). GTX100444
Anti-p21 Cip1 antibody used in Western Blot (WB). GTX100444

Cat. No. GTX100444

Reference 16
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all p21 Cip1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HCT116 treated with 30uM cisplatin for 24hr

Predict Reactivity

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p21 Cip1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100444

GTX100444 ICC/IF Image

p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX100444) diluted at 1:1000.
Red: Histone H3S10ph (phospho Ser10), a nucleus marker, stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-p21 Cip1 antibody used in Western Blot (WB). GTX100444

GTX100444 WB Image

Orthogonal

Sample (30 μg of whole cell lysate)
A: HCT116
B: HCT116 treated with 30 μM cisplatin for 24 hr
12% SDS PAGE
GTX100444 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p21 Cip1 antibody used in Western Blot (WB). GTX100444

GTX100444 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

REFERENCE

Application Reference

Oyama Y et al. PLoS One 2021; 16 (6) : e0251079 CHD4 regulates platinum sensitivity through MDR1 expression in ovarian cancer: A potential role of CHD4 inhibition as a combination therapy with platinum agents.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Park SH et al. FASEB J 2018; : fj201800011R resistance to * and cross-resistance to irreversible egfr-tkis mediated by disruption of the keap1-nrf2 pathway in human lung cancer cells.
Application : WB
Lin KT et al. Am J Chin Med 2018; : 43116 Rhodiola crenulata Attenuates γ-Ray Induced Cellular Injury Via Modulation of Oxidative Stress in Human Skin Cells.
Application : WB
Reactivity : Human
Chiu YS et al. Sci Rep 2017; 7 (1) : 17609 Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

p21 Cip1 antibody Cat. No. GTX100444

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-p21 Cip1 antibody used in Western Blot (WB). GTX100444
Date : Anonymous submitted on 17-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Oyama Y et al. PLoS One 2021; 16 (6):e0251079 CHD4 regulates platinum sensitivity through MDR1 expression in ovarian cancer: A potential role of CHD4 inhibition as a combination therapy with platinum agents.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Park SH et al. FASEB J 2018;:fj201800011R resistance to * and cross-resistance to irreversible egfr-tkis mediated by disruption of the keap1-nrf2 pathway in human lung cancer cells.
Application : WB
Lin KT et al. Am J Chin Med 2018;:43116 Rhodiola crenulata Attenuates γ-Ray Induced Cellular Injury Via Modulation of Oxidative Stress in Human Skin Cells.
Application : WB
Reactivity : Human
Chiu YS et al. Sci Rep 2017; 7 (1):17609 Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma.
Application : WB
Reactivity : Human
Briest F et al. Neuroendocrinology 2017; (Epub) Mechanisms of targeting the MDM2-p53-FOXM1 axis in well-differentiated intestinal neuroendocrine tumors.
Application : WB
Reactivity : Human
Salazar G et al. Free Radic Biol Med 2017; 108:225-235 Zinc regulates Nox1 expression through a NF-κB and mitochondrial ROS dependent mechanism to induce senescence of vascular smooth muscle cells.
Application : WB
Reactivity : Rat
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Xu YM et al. Oncotarget 2016; 7 (5):6146-58 Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Chen CC et al. PLoS One 2016; 11 (12):e0168092 Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Application : WB
Reactivity : Human
Feresin RG et al. Food Funct 2016; Blackberry, raspberry and black raspberry polyphenol extracts attenuate angiotensin II-induced senescence in vascular smooth muscle cells.
Application : WB
Reactivity : Rat
So EC et al. Onco Targets Ther 2016; 9:2519-33 Midazolam regulated caspase pathway, endoplasmic reticulum stress, autophagy, and cell cycle to induce apoptosis in MA-10 mouse Leydig tumor cells.
Rodriguez A et al. Theor Biol Med Model 2015; 12:19 Fanconi anemia cells with unrepaired DNA damage activate components of the checkpoint recovery process.
Application : WB
Chang MC et al. PLoS One 2015; 10 (12):e0143663 Effects of Camphorquinone on Cytotoxicity, Cell Cycle Regulation and Prostaglandin E2 Production of Dental Pulp Cells: Role of ROS, ATM/Chk2, MEK/ERK and Hemeoxygenase-1.
Application : WB
Reactivity : Human
Chen YJ et al. Life Sci 2013; 92 (22):1081-92 The synthetic flavonoid WYC02-9 inhibits colorectal cancer cell growth through ROS-mediated activation of MAPK14 pathway.
Wang SH et al. J Cell Physiol 2012; 227 (8):3063-71 Ganoderma lucidum polysaccharides prevent platelet-derived growth factor-stimulated smooth muscle cell proliferation in vitro and neointimal hyperplasia in the endothelial-denuded artery in vivo.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169