GeneTex
United States (US)

p21 Cip1 antibody

Orthogonal
GTX100444_40996_20150518_IFA_19011506_154.jpg
GTX100444_40996_WB_19011506_713.jpg

Cat No. GTX100444

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HCT116 treated with 30uM cisplatin for 12hr , HCT116 treated with 30uM cisplatin for 24hr , *HUVEC

Predict Reactivity

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase Inhibitor 1A,Cap20,Cdkn1,Cip1,Mda-6,P21,Sdi1,Waf1,P21Cip1,Cdkn1A

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100444_40996_20150518_IFA_19011506_154.jpg

GTX100444 ICC/IF Image

p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX100444) diluted at 1:1000.
Red: Histone H3S10ph (phospho Ser10), a nucleus marker, stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100444_40996_WB_19011506_713.jpg

GTX100444 WB Image

Orthogonal

Sample (30 µg of whole cell lysate)
A: HCT116
B: HCT116 treated with 30 µM cisplatin for 24 hr
12% SDS PAGE
GTX100444 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

p21 Cip1 antibody Cat No. GTX100444

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-Nov-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf