Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody (GTX112898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

p21 antibody detects p21 protein by Western blot analysis.
A. 30 μg HCT116 whole cell extract (untreated)
B. 30 μg HCT116 whole cell extract (30 μM Cisplatin treatment for 24 h)
C. 30 μg HCT116 whole cell extract (30 μM Cisplatin treatment for 48 h)
12 % SDS-PAGE
p21 antibody (GTX112898) dilution: 1:1000

p21 Cip1 antibody detects CDKN1A protein at nucleus on SkHep1xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1xenograft.
p21 Cip1 antibody (GTX112898) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX112898) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human normal colon tissue.
p21 Cip1 stained by p21 Cip1 antibody (GTX112898) diluted at 1:500.
Antigen Retrieval: EDTA buffer, 20 min

The data was published in the journal Front Genet in 2020. PMID: 32158470

The data was published in the journal Front Genet in 2020. PMID: 32158470

The data was published in the journal Front Physiol in 2018.PMID: 30333755

The data was published in the journal Cell Death Dis in 2018.PMID: 29511169

The data was published in the journal Cell Death Dis in 2018.PMID: 29511169