GeneTex
United States (US)

p21 Cip1 antibody

KO/KD Orthogonal
GTX112898_40086_WB_HCT116 cisplatin_18121410_262.jpg
GTX112898_41626_20160113_IFA_18121410_715.jpg
GTX112898_40086_20170413_WB_KO_watermark_18121410_690.jpg
GTX112898_41010_IHC_18121410_365.jpg

Cat No. GTX112898

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HeLa , HCT116 treated 30uM cisplatin 24hr

Predict Reactivity

Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

wild-type p53-activated fragment 1 antibody, p21CIP1 antibody, melanoma differentiation associated protein 6 antibody, cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody, cyclin-dependent kinase inhibitor 1 antibody, WAF1 antibody, SDI1 antibody, P21 antibody, MDA-6 antibody, DNA synthesis inhibitor antibody, CIP1 antibody, CDKN1A antibody, CDKN1 antibody, CDK-interaction protein 1 antibody, CDK-interacting protein 1 antibody, CAP20 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX112898_40086_WB_HCT116 cisplatin_18121410_262.jpg

GTX112898 WB Image

Orthogonal

Sample (30 µg of whole cell lysate)
A: HCT116 cells with 30 µM cisplatin treatment for 0 hr
B: HCT116 cells with 30 µM cisplatin treatment for 24 hr
12% SDS PAGE
GTX112898 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112898_41626_20160113_IFA_18121410_715.jpg

GTX112898 ICC/IF Image

p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX112898) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX112898_40086_20170413_WB_KO_watermark_18121410_690.jpg

GTX112898 WB Image

KO/KD

Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody (GTX112898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX112898_41010_IHC_18121410_365.jpg

GTX112898 IHC-P Image

p21 Cip1 antibody detects CDKN1A protein at nucleus on SkHep1xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1xenograft.
p21 Cip1 antibody (GTX112898) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf