GeneTex
United States (US)

p21 Cip1 antibody

Cat No. GTX14975

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

WB: Use at a dilution of 1/250 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 19.7 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

18 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.02mM Sodium Azide; Constituents: 40% Glycerol, PBS. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: GRKRRQTSMTDFYHSKRRLIFSKRKP , corresponding to amino acids 139-164 of Human p21.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase Inhibitor 1A,Cap20,Cdkn1,Cip1,Mda-6,P21,Sdi1,Waf1,P21Cip1,Cdkn1A

Cellular Localization

Nuclear

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Database

Research Area