GeneTex
United States (US)

p21 Cip1 antibody

p21-antibody-GTX27960-WB-1_18121411_318.jpg

Cat No. GTX27960

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

IHC-Fr: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Can be blocked with GTX28015 p21 (C-term) peptide (human) . Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

18 kDa. ( Note )

Positive Control

C32

Specificity/Sensitivity

Reacts with p21(also designated WAF1/Cip1)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (Human) (C terminal).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase Inhibitor 1A,Cap20,Cdkn1,Cip1,Mda-6,P21,Sdi1,Waf1,P21Cip1,Cdkn1A

Cellular Localization

Nuclear

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks (1-3). p21 (WAF1/Cip1) inhibits the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

Database

Research Area

DATA IMAGES
p21-antibody-GTX27960-WB-1_18121411_318.jpg

GTX27960 WB Image

Primary antibody (at 1:200) used to detect p21 expression by western blot. Samples 15µg each of A) A431 lysate B) HL-60 lysate C) Hela lysate

REFERENCE