WB analysis of EGF-treated HT1080 (A), EGF-treated HeLa (B) whole cell lysates using GTX32376 p21 Cip1 (phospho Thr145) antibody.

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX32376 p21 Cip1 (phospho Thr145) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

ICC/IF analysis of formalin-fixed HeLa cells using GTX32376 p21 Cip1 (phospho Thr145) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes