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p22phox antibody, Internal

Anti-p22phox antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX46749
Anti-p22phox antibody, Internal used in Western Blot (WB). GTX46749

Cat. No. GTX46749

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($409)
See all p22phox products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

human fetal small intestine

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human p22phox.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome b-245 alpha chain , p22-PHOX

Cellular Localization

Cell membrane

Background

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p22phox antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX46749

GTX46749 IHC-P Image

IHC-P analysis of human heart tissue using GTX46749 CYBA antibody at 1:100. Left to right:DAPI, CYBA, Merge. Heat induced antigen retrieval method using Antigen retrieval buffer (Tris EDTA, pH 9.0) for 2-3 minutes in pressure cooker.

Anti-p22phox antibody, Internal used in Western Blot (WB). GTX46749

GTX46749 WB Image

WB analysis of human fetal small intestine tissue using GTX46749 CYBA antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409