GeneTex

  • Country / Location Selection

United States (US)

p22phox antibody

Comparison Orthogonal
Anti-p22phox antibody used in Western Blot (WB). GTX133970
Anti-p22phox antibody used in IHC (Paraffin sections) (IHC-P). GTX133970

Cat. No. GTX133970

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all p22phox products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human p22phox. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887158

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome b-245 alpha chain , p22-PHOX

Cellular Localization

Membrane

Background

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p22phox antibody used in Western Blot (WB). GTX133970

GTX133970 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with p22phox antibody (GTX133970) diluted at 1:1000 and competitor's antibody (sc-20781) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Santa Cruz Biotechnology is not affiliated with GeneTex and does not endorse this product.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-p22phox antibody used in IHC (Paraffin sections) (IHC-P). GTX133970

GTX133970 IHC-P Image

p22phox antibody detects p22phox protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p22phox stained by p22phox antibody (GTX133970) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Miyano K et al. Redox Biol 2022; 56 : 102479 Regulation of Derlin-1-mediated degradation of NADPH oxidase partner p22(phox) by thiol modification.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for p22phox antibody (GTX133970). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169