p23 antibody [JJ3]

Cat. No. GTX22814

Host	Mouse
Clonality	Monoclonal
Clone Name	JJ3
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, IP, IHC
Reactivity	Human, Mouse, Rat, Rabbit, Chicken, Guinea pig
Package	100 μl ($399)

See all p23 products

Datasheet
Document

View product citations for antibody GTX22814 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ICC/IF	1:200-1:2000
IHC-P	Assay dependent
FACS	1:100
IP	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human p23 protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prostaglandin E synthase 3 , P23 , TEBP , cPGES

Cellular Localization

Cytoplasm

Background

This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes. [provided by RefSeq, Feb 2016]

Database

Gene ID: 10728 PTGES3
UniProt: Q15185 PTGES3

Research Area

DATA IMAGES

GTX22814 FACS Image

FACS analysis of Jurkat cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX22814 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22814 p23 antibody [JJ3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

GTX22814 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:500 overnight at 4ºC

GTX22814 IHC-P Image

IHC-P analysis of human testis tissue using GTX22814 p23 antibody [JJ3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

GTX22814 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:500 overnight at 4ºC

GTX22814 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22814 p23 antibody [JJ3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:500 overnight at 4ºC

GTX22814 FACS Image

FACS analysis of 3T3 cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX22814 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX22814 p23 antibody [JJ3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

GTX22814 FACS Image

FACS analysis of Hela cells using GTX22814 p23 antibody [JJ3] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX22814 WB Image

WB analysis of 25 ug of Hela (Lane 1), HepG2 (Lane 2), and mouse spleen cell lysates (Lane 3) using GTX22814 p23 antibody [JJ3].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

p23 antibody [JJ3]

Cat. No. GTX22814

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX22814 FACS Image

GTX22814 IHC-P Image

GTX22814 ICC/IF Image

GTX22814 IHC-P Image

GTX22814 ICC/IF Image

GTX22814 ICC/IF Image

GTX22814 FACS Image

GTX22814 IHC-P Image

GTX22814 FACS Image

GTX22814 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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