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p23 antibody [N1C3-2]

Anti-p23 antibody [N1C3-2] used in Western Blot (WB). GTX112655
Anti-p23 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX112655
Anti-p23 antibody [N1C3-2] used in Western Blot (WB). GTX112655
Anti-p23 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112655
Anti-p23 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX112655
Anti-p23 antibody [N1C3-2] used in Western Blot (WB). GTX112655

Cat. No. GTX112655

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

Mouse brain , A431 , H1299 , HeLa

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human p23 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951527

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prostaglandin E synthase 3 , P23 , TEBP , cPGES

Cellular Localization

Cytoplasm

Background

Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.

Database

Research Area

DATA IMAGES

Anti-p23 antibody [N1C3-2] used in Western Blot (WB). GTX112655

GTX112655 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
12% SDS PAGE
GTX112655 diluted at 1:1000

Anti-p23 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX112655

GTX112655 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HT-29 xenograft, using p23(GTX112655) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p23 antibody [N1C3-2] used in Western Blot (WB). GTX112655

GTX112655 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112655 diluted at 1:1000

Anti-p23 antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112655

GTX112655 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using P23 (GTX112655) antibody at 1:200 dilution.

Anti-p23 antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX112655

GTX112655 IHC-P Image

p23 antibody [N1C3-2] detects p23 protein at cytoplasm in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
p23 antibody [N1C3-2] (GTX112655) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p23 antibody [N1C3-2] used in Western Blot (WB). GTX112655

GTX112655 WB Image

The data was published in the journal Mol Neurodegener in 2015. PMID: 26306916

REFERENCE

REVIEW

p23 antibody [N1C3-2] Cat. No. GTX112655

Rating ( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 3.5 based on 2 users reviews)
Date : Anonymous submitted on 08-Jul-2013
Score :
Application Tested : WB
Sample Species : Rat
Sample : RK3E cells
Amount used : 50μg
Blocking : 5% not-fat milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 11-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 35μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169