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p23 antibody [N1C3]

KO/KD
Anti-p23 antibody [N1C3] used in Western Blot (WB). GTX106550
Anti-p23 antibody [N1C3] used in Western Blot (WB). GTX106550
Anti-p23 antibody [N1C3] used in Western Blot (WB). GTX106550
Anti-p23 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106550

Cat. No. GTX106550

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all p23 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T , Mouse brain , 293T

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p23. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729207

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prostaglandin E synthase 3 , P23 , TEBP , cPGES

Cellular Localization

Cytoplasm

Background

Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.

Database

Research Area

DATA IMAGES

Anti-p23 antibody [N1C3] used in Western Blot (WB). GTX106550

GTX106550 WB Image

KO/KD

Wild-type (WT) and p23 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p23 antibody [N1C3] (GTX106550) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p23 antibody [N1C3] used in Western Blot (WB). GTX106550

GTX106550 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX106550 diluted at 1:10000

Anti-p23 antibody [N1C3] used in Western Blot (WB). GTX106550

GTX106550 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX106550 diluted at 1:5000

Anti-p23 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX106550

GTX106550 IHC-P Image

p23 antibody [N1C3] detects p23 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
p23 antibody [N1C3] (GTX106550) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169