APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.5-1.5μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89293-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-EQTPKKPGLRRRQT, from the C Terminus of the protein sequence according to NP_004055.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1
Cellular Localization
Nucleus , Cytoplasm , Endosome
Background
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
Database
Research Area
DATA IMAGES
|
GTX89293 WB Image
WB analysis of human breast lysate using GTX89293 p27 Kip1 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for p27 Kip1 antibody, C-term (GTX89293). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p27 Kip1 antibody, C-term (GTX89293). Be the first to share your experience with this product.
![Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214](/upload/website/prouct_img/small/GTX70214/GTX70214_41848_20160623_WB_shRNA_watermark_18121411_112.jpg "Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214")
