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p27 Kip1 antibody [DCS-72.F6]

Anti-p27 Kip1 antibody [DCS-72.F6] used in IHC (Paraffin sections) (IHC-P). GTX34931
Anti-p27 Kip1 antibody [DCS-72.F6] used in IHC (Paraffin sections) (IHC-P). GTX34931
Anti-p27 Kip1 antibody [DCS-72.F6] used in Flow cytometry (FACS). GTX34931

Cat No. GTX34931

Host

Mouse

Clonality

Monoclonal

Clone Name

DCS-72.F6

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Mouse

Immunogen

Recombinant full-length Mouse p27 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus,Cytoplasm,Endosome

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 antibody [DCS-72.F6] used in IHC (Paraffin sections) (IHC-P). GTX34931

GTX34931 IHC-P Image

IHC-P analysis of human colon cancer tissue using GTX34931 p27 Kip1 antibody [DCS-72.F6].

Anti-p27 Kip1 antibody [DCS-72.F6] used in IHC (Paraffin sections) (IHC-P). GTX34931

GTX34931 IHC-P Image

IHC-P analysis of human cervical cancer tissue using GTX34931 p27 Kip1 antibody [DCS-72.F6].

Anti-p27 Kip1 antibody [DCS-72.F6] used in Flow cytometry (FACS). GTX34931

GTX34931 FACS Image

FACS analysis of HeLa cells using GTX34931 p27 Kip1 antibody [DCS-72.F6].
Black : Blank
Grey : Isotype Control
Green : Primary antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf