APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human p27 Kip1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1
Cellular Localization
Nucleus,Cytoplasm,Endosome
Background
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
Database
Research Area
DATA IMAGES
![Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869](/templates/default/js/lightbox/images/loading.gif "Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869")
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GTX638869 WB Image
Wild-type (WT) and CDKN1B knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638869 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638869 ICC/IF Image
p27 Kip1 antibody [HL2508] detects p27 Kip1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p27 Kip1 stained by p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638869 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with p27 Kip1 antibody [HL2508] (GTX638869) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for p27 Kip1 antibody [HL2508] (GTX638869). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p27 Kip1 antibody [HL2508] (GTX638869). Be the first to share your experience with this product.
![Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869](/upload/website/prouct_img/small/GTX638869/GTX638869_T-45117_20230811_WB_KO_watermark_23081619_359.webp "Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869")
![Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869](/upload/website/prouct_img/small/GTX638869/GTX638869_45194_20231013_WB_TPM_watermark_23101921_530.webp "Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869")
![Anti-p27 Kip1 antibody [HL2508] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638869](/upload/website/prouct_img/small/GTX638869/GTX638869_T-45117_20231006_ICC_IF_23111422_788.webp "Anti-p27 Kip1 antibody [HL2508] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638869")
![Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869](/upload/website/prouct_img/small/GTX638869/GTX638869_T-45117_20230825_WB_23083020_155.webp "Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869")
![Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869](/upload/website/prouct_img/normal/GTX638869/GTX638869_T-45117_20230811_WB_KO_watermark_23081619_359.webp "Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869")
![Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869](/upload/website/prouct_img/normal/GTX638869/GTX638869_45194_20231013_WB_TPM_watermark_23101921_530.webp "Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869")
![Anti-p27 Kip1 antibody [HL2508] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638869](/upload/website/prouct_img/normal/GTX638869/GTX638869_T-45117_20231006_ICC_IF_23111422_788.webp "Anti-p27 Kip1 antibody [HL2508] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638869")
![Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869](/upload/website/prouct_img/normal/GTX638869/GTX638869_T-45117_20230825_WB_23083020_155.webp "Anti-p27 Kip1 antibody [HL2508] used in Western Blot (WB). GTX638869")