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p27 Kip1 antibody, Internal

Anti-p27 Kip1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89392
Anti-p27 Kip1 antibody, Internal used in Western Blot (WB). GTX89392

Cat No. GTX89392

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Prostate shows nuclear staining of secretory cells.

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89392-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the protein sequence according to NP_004055.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cyclin Dependent Kinase Inhibitor 1B , Cdkn4 , Kip1 , Men1B , Men4 , P27Kip1 , Cdkn1B

Cellular Localization

Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation t

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89392

GTX89392 IHC-P Image

IHC-P analysis of human prostate using GTX89392 p27 Kip1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-p27 Kip1 antibody, Internal used in Western Blot (WB). GTX89392

GTX89392 WB Image

WB analysis of human prostate lysate using GTX89392 p27 Kip1 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf