*Optimal dilutions/concentrations should be determined by the researcher.
Human Prostate shows nuclear staining of secretory cells.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX89392-PEP).
Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the protein sequence according to NP_004055.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1
Nucleus , Cytoplasm , Endosome
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
GTX89392 IHC-P Image
IHC-P analysis of human prostate using GTX89392 p27 Kip1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
GTX89392 WB Image
WB analysis of human prostate lysate using GTX89392 p27 Kip1 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer