APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 0.25-0.5μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human CDKN1B protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CDKN1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1 , cyclin dependent kinase inhibitor 1B , p27 kip1
Cellular Localization
Nucleus,Cytoplasm,Endosome
Background
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
Database
Research Area
DATA IMAGES
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/templates/default/js/lightbox/images/loading.gif "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
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GTX34930 IHC-P Image
IHC-P analysis of human cervical carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].
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GTX34930 FACS Image
FACS analysis of Jurkat cells using GTX34930 p27 Kip1 antibody [KIP1/769].
Black : Blank
Grey : Isotype Control
Green : Primary antibody
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GTX34930 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].
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GTX34930 IHC-P Image
IHC-P analysis of rat colon carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].
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GTX34930 ICC/IF Image
ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34930 p27 Kip1 antibody [KIP1/769].
Green : Primary antibody
Red : Phalloidin
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GTX34930 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].
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REFERENCE
There are currently no references for p27 Kip1 antibody [KIP1/769] (GTX34930). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p27 Kip1 antibody [KIP1/769] (GTX34930). Be the first to share your experience with this product.
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/small/GTX34930/GTX34930_20200115_IHC-P_429_w_23060801_975.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in Flow cytometry (FACS). GTX34930](/upload/website/prouct_img/small/GTX34930/GTX34930_20200115_FACS_1469_w_23060801_771.webp "Anti-p27 Kip1 antibody [KIP1/769] used in Flow cytometry (FACS). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/small/GTX34930/GTX34930_20200115_IHC-P_1039_w_23060801_427.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/small/GTX34930/GTX34930_20200115_IHC-P_1852_w_23060801_307.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34930](/upload/website/prouct_img/small/GTX34930/GTX34930_20200115_ICC-IF_1735_w_23060801_182.webp "Anti-p27 Kip1 antibody [KIP1/769] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/small/GTX34930/GTX34930_20200115_IHC-P_486_w_23060801_744.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/normal/GTX34930/GTX34930_20200115_IHC-P_429_w_23060801_975.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in Flow cytometry (FACS). GTX34930](/upload/website/prouct_img/normal/GTX34930/GTX34930_20200115_FACS_1469_w_23060801_771.webp "Anti-p27 Kip1 antibody [KIP1/769] used in Flow cytometry (FACS). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/normal/GTX34930/GTX34930_20200115_IHC-P_1039_w_23060801_427.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/normal/GTX34930/GTX34930_20200115_IHC-P_1852_w_23060801_307.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34930](/upload/website/prouct_img/normal/GTX34930/GTX34930_20200115_ICC-IF_1735_w_23060801_182.webp "Anti-p27 Kip1 antibody [KIP1/769] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34930")
![Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930](/upload/website/prouct_img/normal/GTX34930/GTX34930_20200115_IHC-P_486_w_23060801_744.webp "Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930")