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p27 Kip1 antibody [KIP1/769]

Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930
Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930
Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930
Anti-p27 Kip1 antibody [KIP1/769] used in Flow cytometry (FACS). GTX34930
Anti-p27 Kip1 antibody [KIP1/769] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34930
Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930

Cat No. GTX34930

Host

Mouse

Clonality

Monoclonal

Clone Name

KIP1/769

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant full-length human CDKN1B protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDKN1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1 , cyclin dependent kinase inhibitor 1B , p27 kip1

Cellular Localization

Nucleus,Cytoplasm,Endosome

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930

GTX34930 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].

Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930

GTX34930 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].

Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930

GTX34930 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].

Anti-p27 Kip1 antibody [KIP1/769] used in Flow cytometry (FACS). GTX34930

GTX34930 FACS Image

FACS analysis of Jurkat cells using GTX34930 p27 Kip1 antibody [KIP1/769].
Black : Blank
Grey : Isotype Control
Green : Primary antibody

Anti-p27 Kip1 antibody [KIP1/769] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34930

GTX34930 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34930 p27 Kip1 antibody [KIP1/769].
Green : Primary antibody
Red : Phalloidin

Anti-p27 Kip1 antibody [KIP1/769] used in IHC (Paraffin sections) (IHC-P). GTX34930

GTX34930 IHC-P Image

IHC-P analysis of rat colon carcinoma tissue using GTX34930 p27 Kip1 antibody [KIP1/769].

REFERENCE

There are currently no references for p27 Kip1 antibody [KIP1/769] (GTX34930). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p27 Kip1 antibody [KIP1/769] (GTX34930). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf