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p27 Kip1 antibody [SPM348]

Anti-p27 Kip1 antibody [SPM348] used in IHC (Paraffin sections) (IHC-P). GTX34929
Anti-p27 Kip1 antibody [SPM348] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34929

Cat No. GTX34929

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM348

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Mouse

Immunogen

Purified GST-p27 fusion protein of Mouse origin

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus,Cytoplasm,Endosome

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 antibody [SPM348] used in IHC (Paraffin sections) (IHC-P). GTX34929

GTX34929 IHC-P Image

IHC-P analysis of human prostate tissue using GTX34929 p27 Kip1 antibody [SPM348].

Anti-p27 Kip1 antibody [SPM348] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34929

GTX34929 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34929 p27 Kip1 antibody [SPM348].
Green : Primary antibody
Red : Phalloidin

REFERENCE

There are currently no references for p27 Kip1 antibody [SPM348] (GTX34929). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p27 Kip1 antibody [SPM348] (GTX34929). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf