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p27 Kip1 antibody [SX53G8]

Anti-p27 Kip1 antibody [SX53G8] used in Flow cytometry (FACS). GTX34928
Anti-p27 Kip1 antibody [SX53G8] used in IHC (Paraffin sections) (IHC-P). GTX34928
Anti-p27 Kip1 antibody [SX53G8] used in IHC (Paraffin sections) (IHC-P). GTX34928

Cat No. GTX34928

Host Mouse
Clonality Monoclonal
Clone Name SX53G8
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse, Rat, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified GST-p27 fusion protein of human origin

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase Inhibitor 1B,Cdkn4,Kip1,Men1B,Men4,P27Kip1,Cdkn1B

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES
Anti-p27 Kip1 antibody [SX53G8] used in Flow cytometry (FACS). GTX34928

GTX34928 FACS Image

Flow Cytometry of human p27 on HeLa Cells.Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled p27 Monoclonal Antibody (SX53G8).

Anti-p27 Kip1 antibody [SX53G8] used in IHC (Paraffin sections) (IHC-P). GTX34928

GTX34928 IHC-P Image

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with p27 Monoclonal Antibody (SX53G8)

Anti-p27 Kip1 antibody [SX53G8] used in IHC (Paraffin sections) (IHC-P). GTX34928

GTX34928 IHC-P Image

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Monoclonal Antibody (SX53G8)

Package List Price ($)
$ 329