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p27 Kip1 antibody

Overexpression
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547
Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547

Cat. No. GTX26547

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Human
Package
100 μl ($589)
See all p27 Kip1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:4000
IP 1:100
ELISA 1:10000-1:40000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

No reactivity is observed with p27 from mouse.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human p27 protein.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1

Cellular Localization

Nucleus,Cytoplasm,Endosome

Background

p27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547

GTX26547 WB Image

Western blot using GeneTex affinity purified anti-p27 antibody (GTX26547) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight™ 800 conjugated Goat anti Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547

GTX26547 WB Image

WB analysis of MCF-7 whole cell lysate using GTX26547 p27 Kip1 antibody.
Dilution : 1:500

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547

GTX26547 WB Image

Overexpression

WB analysis of non-transfected and transfected 293T whole cell lysate using GTX26547 p27 Kip1 antibody.
Loading : 30 μg
Dilution : 1:2000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589