GeneTex
  • Country / Location Selection

United States (US)

p27 Kip1 antibody

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547

Cat No. GTX26547

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Human
Package
100 μl ($459)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:4000
IP 1:100
ELISA 1:10000 - 1:40000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

No reactivity is observed with p27 from mouse.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

80 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length recombinant human p27 protein.

Purification

Antiserum
This product was prepared from monospecific antiserum by delipidation and defibrination.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1

Cellular Localization

Nucleus,Cytoplasm,Endosome

Background

p27 Kip is a cyclin-dependent kinase inhibitor that shares a limited similarity with CDK inhibitor CDKN1A/p21. p27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 antibody used in Western Blot (WB). GTX26547

GTX26547 WB Image

Western blot using GeneTex affinity purified anti-p27 antibody (GTX26547) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight™ 800 conjugated Goat anti Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

REFERENCE

There are currently no references for p27 Kip1 antibody (GTX26547). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p27 Kip1 antibody (GTX26547). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf