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p27 Kip1 antibody

Cat No. GTX27961

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, IHC

Reactivity

Human, Mouse, Rat, Sheep
Package
1 ml ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX28016).

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1 % sodium azide, 0.2% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide mapping to the carboxy terminus of human p27.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_369312

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1

Cellular Localization

Nucleus , Cytoplasm , Endosome

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Database

Research Area

REFERENCE

REVIEW

p27 Kip1 antibody Cat No. GTX27961

Rating ( Average 5 based on 1 users reviews)
Application
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC
Sample Species : Sheep
Figure Note : The data was peer reviewed and published in the journal Mol Cell Endocrinol in 2013.PMID: 23791816