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p27 Kip1 (phospho Ser10) antibody

Anti-p27 Kip1 (phospho Ser10) antibody used in Western Blot (WB). GTX17353
Anti-p27 Kip1 (phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX17353

Cat No. GTX17353

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
400 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Mouse, Hamster(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27Kip1.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1

Cellular Localization

Nucleus,Cytoplasm,Endosome

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 (phospho Ser10) antibody used in Western Blot (WB). GTX17353

GTX17353 WB Image

WB analysis of HL-60 cell lysate using GTX17353 p27 Kip1 (phospho Ser10) antibody.

Anti-p27 Kip1 (phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX17353

GTX17353 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX17353 p27 Kip1 (phospho Ser10) antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf