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p27 Kip1 (phospho Thr198) antibody

Anti-p27 Kip1 (phospho Thr198) antibody used in Western Blot (WB). GTX55090

Cat No. GTX55090

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of p27 Kip1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1

Cellular Localization

Nucleus,Cytoplasm,Endosome

Background

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-p27 Kip1 (phospho Thr198) antibody used in Western Blot (WB). GTX55090

GTX55090 WB Image

WB analysis of EGF-treated HEK293T (A), MCF7 (B), NIH3T3 (C), EGF-treated Raw264.7 (D), EGF-treated H9C2 (E) whole cell lysates using GTX55090 p27 Kip1 (phospho Thr198) antibody.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf