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p35 antibody

Cat No. GTX10570

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Rat
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

rat brain cytosol
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

synthetic peptide corresponding to the C-terminus of p35 (Cdk5R), (human, amino acids 192-209).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cyclin dependent kinase 5 regulatory subunit 1 , CDK5P35 , CDK5R , NCK5A , p23 , p25 , p35 , p35nck5a

Background

p35 / nck5a, is a neuron-specific activator of Cdk 5. Treatment of cultured neurons with cyclohexamide induces the rapid disappearance of p35 accompanied by parallel inactivation of the kinase activity of Cdk 5. The disappearance of p35 is blocked with proteasome inhibitors, indicating the involvement of proteasome. The degradation of p35 is induced with okadaic acid in the presence of ATP in neuron extracts. The degradation of p35 by proteasome in cultured neurons is stimulated by okadaic acid in the absence of cyclohexamide. Cdk 5 and its neuron specific regulator p35 are essential for neuronal migration and for the laminar configuration of the cerebral cortex. p35 / Cdk 5 kinase concentrates at the leading edges of axonal growth cones and regulates neurite outgrowth in cortical neurons in culture. p35 / Cdk 5 kinase co-localizes with Rac in neuronal growth cones.

Database

Research Area

REFERENCE
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