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p35 antibody

Anti-p35 antibody used in IHC (Paraffin sections) (IHC-P). GTX133713
Anti-p35 antibody used in Western Blot (WB). GTX133713
Anti-p35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133713
Anti-p35 antibody used in IHC (Paraffin sections) (IHC-P). GTX133713

Cat No. GTX133713

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human p35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887072

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 5 regulatory subunit 1 , CDK5P35 , CDK5R , NCK5A , p23 , p25 , p35 , p35nck5a

Cellular Localization

Cyclin-dependent kinase 5 activator 1 , p35: Cell membrane; Lipid-anchor; Cytoplasmic side , p25: Nucleus , Cytoplasm , perinuclear region , Cyclin-dependent kinase 5 activator 1 , p35: Cell membrane , p25: Nucleus , Cytoplasm , perinuclear region

Background

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p35 antibody used in IHC (Paraffin sections) (IHC-P). GTX133713

GTX133713 IHC-P Image

p35 antibody detects p35 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
p35 stained by p35 antibody (GTX133713) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p35 antibody used in Western Blot (WB). GTX133713

GTX133713 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with p35 antibody (GTX133713) diluted at 1:1000 and competitor's antibody (SC-820) diluted by 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-p35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133713

GTX133713 ICC/IF Image

p35 antibody detects p35 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p35 stained by p35 antibody (GTX133713) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p35 antibody used in IHC (Paraffin sections) (IHC-P). GTX133713

GTX133713 IHC-P Image

p35 antibody detects p35 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p35 stained by p35 antibody (GTX133713) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169