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p35 antibody

Anti-p35 antibody used in Western Blot (WB). GTX34250
Anti-p35 antibody used in Western Blot (WB). GTX34250

Cat No. GTX34250

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Specificity/Sensitivity

p35 Polyclonal Antibody detects endogenous levels of p35 protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide derived from the N-terminal region of human p35 at AA range: 30-110.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 5 Regulatory Subunit 1 , Cdk5P35 , Cdk5R , Nck5A , P23 , P25 , P35 , P35Nck5A , Cdk5R1

Background

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-p35 antibody used in Western Blot (WB). GTX34250

GTX34250 WB Image

Western Blot (WB) analysis of HeLa cells using p35 Polyclonal Antibody.

Anti-p35 antibody used in Western Blot (WB). GTX34250

GTX34250 WB Image

Western Blot (WB) analysis of specific cells using p35 Polyclonal Antibody.

REFERENCE
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