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p35 antibody

Anti-p35 antibody used in Western Blot (WB). GTX34250
Anti-p35 antibody used in Western Blot (WB). GTX34250

Cat No. GTX34250

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthesized peptide derived from human p35 at 30-110 aa, N-terminal.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CDK5P35,CDK5R,CDK5R1,NCK5A,cyclin dependent kinase 5 regulatory subunit 1,p23,p25,p35,p35nck5a

Cellular Localization

Cell membrane,Nucleus,Cytoplasm,Perinuclear region

Background

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p35 antibody used in Western Blot (WB). GTX34250

GTX34250 WB Image

WB analysis of K562 cell lysate using GTX34250 p35 antibody.

Anti-p35 antibody used in Western Blot (WB). GTX34250

GTX34250 WB Image

WB analysis of HeLa cell lysate using GTX34250 p35 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319