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p37 antibody [1320]

Anti-p37 antibody [1320] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70285
Anti-p37 antibody [1320] used in Western Blot (WB). GTX70285

Cat No. GTX70285

Host

Mouse

Clonality

Monoclonal

Clone Name

1320

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encoding the full-length p37 subunit ofhuman RFC purified from E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Replication Factor C Subunit 4 , A1 , Rfc37 , Rfc4

Cellular Localization

Nuclear

Background

Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9.

Database

Research Area

DATA IMAGES

Anti-p37 antibody [1320] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70285

GTX70285 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320](GTX70285) antibody at 1:200 dilution.

Anti-p37 antibody [1320] used in Western Blot (WB). GTX70285

GTX70285 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p37 antibody [1320] (GTX70285) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329