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p37 antibody [1320]

Anti-p37 antibody [1320] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70285
Anti-p37 antibody [1320] used in Western Blot (WB). GTX70285

Cat. No. GTX70285

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1320

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all RFC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encoding the full-length p37 subunit ofhuman RFC purified from E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372906

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 4 , A1 , RFC37

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p37 antibody [1320] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70285

GTX70285 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320](GTX70285) antibody at 1:200 dilution.

Anti-p37 antibody [1320] used in Western Blot (WB). GTX70285

GTX70285 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p37 antibody [1320] (GTX70285) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wei L et al. Nat Commun 2021; 12 (1) : 1591 Hepatitis B virus cccDNA is formed through distinct repair processes of each strand.
Application : WB
Reactivity : Human
Wei L et al. Nat Microbiol 2020; (Epub) Core components of DNA lagging strand synthesis machinery are essential for hepatitis B virus cccDNA formation.
Application : WB
Reactivity : Human
Guo Z et al. Nucleic Acids Res 2009; 37 (10) : 3431-41 Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Application : WB
Reactivity : Mouse
Levin DS et al. J Biol Chem 2004; 279 (53) : 55196-201 A conserved interaction between the replicative clamp loader and DNA ligase in eukaryotes: implications for Okazaki fragment joining.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations