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p37 antibody

Anti-p37 antibody used in Western Blot (WB). GTX70287
Anti-p37 antibody used in Western Blot (WB). GTX70287
Anti-p37 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70287

Cat No. GTX70287

Host

Mouse

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

For WB: Use at a diltuion of 1:2, 000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Not tested in other species. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

40 kDa. ( Note )

Positive Control

HepG2, LNCAP, MCF7, SKOV3

PROPERTIES

Form

Liquid

Buffer

Antiserum diluted in PBS pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant protein encoding the full-length p37 subunit ofhuman RFC purified from E. coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Replication Factor C Subunit 4 , A1 , Rfc37 , Rfc4

Cellular Localization

Nuclear

Background

Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9.

Database

Research Area

DATA IMAGES

Anti-p37 antibody used in Western Blot (WB). GTX70287

GTX70287 WB Image

Anti-p37 antibody used in Western Blot (WB). GTX70287

GTX70287 WB Image

Detection of human p37 protein in HeLa whole cell lysate using GeneTex p37 polyclonal antiserum GTX70287.

Anti-p37 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70287

GTX70287 ICC/IF Image

p37 antibody detects p37 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p37 protein stained by p37 antibody (GTX70287) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 329