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p37 antibody

Anti-p37 antibody used in Western Blot (WB). GTX70287
Anti-p37 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70287
Anti-p37 antibody used in Western Blot (WB). GTX70287

Cat. No. GTX70287

Host

 Mouse

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all RFC4 products

APPLICATION

Application Note

For WB: Use at a diltuion of 1:2, 000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Not tested in other species. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

40 kDa. ( Note )

Positive Control

HepG2, LNCAP, MCF7, SKOV3

PROPERTIES

Form

Liquid

Buffer

Serum diluted with PBS pH7.4

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant protein encoding the full-length p37 subunit ofhuman RFC purified from E. coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_372908

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 4 , A1 , RFC37

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p37 antibody used in Western Blot (WB). GTX70287

GTX70287 WB Image

Anti-p37 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70287

GTX70287 ICC/IF Image

p37 antibody detects p37 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p37 protein stained by p37 antibody (GTX70287) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-p37 antibody used in Western Blot (WB). GTX70287

GTX70287 WB Image

Detection of human p37 protein in HeLa whole cell lysate using GeneTex p37 polyclonal antiserum GTX70287.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399