p38 MAPK antibody [8G4D11]

Cat. No. GTX60771

Host	Mouse
Clonality	Monoclonal
Clone Name	8G4D11
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μg ($409)

See all p38 MAPK products

Datasheet
Document

View product citations for antibody GTX60771 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/75
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human p38 MAPK (AA: 299-360) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1432 MAPK14
UniProt: Q16539 MAPK14

Research Area

DATA IMAGES

GTX60771 ELISA Image

ELISA analysis of antigen using GTX60771 p38 MAPK antibody [8G4D11].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60771 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60771 p38 MAPK antibody [8G4D11].

GTX60771 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60771 p38 MAPK antibody [8G4D11].

GTX60771 FACS Image

FACS analysis of HeLa cells using GTX60771 p38 MAPK antibody [8G4D11].
Green : p38 MAPK
Red : negative control

GTX60771 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60771 p38 MAPK antibody [8G4D11].
Green : p38 MAPK
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60771 WB Image

WB analysis of HeLa (1), NIH3T3 (2), Jurkat (3), Raw264.7 (4), PC-12 (5), C6 (6) and COS7 (7) cell lysate using GTX60771 p38 MAPK antibody [8G4D11].

GTX60771 WB Image

WB analysis of HEK293 (1) and p38 MAPK (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate using GTX60771 p38 MAPK antibody [8G4D11].

REFERENCE

There are currently no references for p38 MAPK antibody [8G4D11] (GTX60771). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for p38 MAPK antibody [8G4D11] (GTX60771). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 409

Qty

p38 MAPK antibody [8G4D11]

Cat. No. GTX60771

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX60771 ELISA Image

GTX60771 IHC-P Image

GTX60771 IHC-P Image

GTX60771 FACS Image

GTX60771 ICC/IF Image

GTX60771 WB Image

GTX60771 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

© 1998-2022 GeneTex, Inc. All rights reserved.

All products are for research use only—Not for use in diagnostic or therapeutic applications.