GeneTex
  • Country / Location Selection

United States (US)

p38 MAPK antibody [N1C3-2]

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

Cat. No. GTX110720

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Sheep, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human p38 MAPK Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617720

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110720

GTX110720 ICC/IF Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720

GTX110720 IHC-P Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720

GTX110720 IHC-P Image

p38 antibody [N1C3-2] detects p38 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
p38 antibody [N1C3-2] (GTX110720) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 29518977

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

The data was published in the journal Mar Drugs in 2019. PMID: 31213027

REFERENCE

REVIEW

p38 MAPK antibody [N1C3-2] Cat. No. GTX110720

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC4 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 5 μM ADC treatment for 0-120 min.
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: PANC1, Lane 2: AsPC1, Lane 3: BxPC3
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169