GeneTex
United States (US)

p38 MAPK antibody [N1C3-2]

KO/KD
GTX110720_40100_20180316_WB_KO_watermark_19011507_304.jpg
GTX110720_40100_20141128_IHC_19011507_209.jpg
GTX110720_40100_20161208_WB_19011507_417.jpg
GTX110720_40100_20151029_IFA_19011507_192.jpg
GTX110720_40100_20160201_IHC-P_R_19011507_903.jpg

Cat No. GTX110720

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Sheep, Bovine, Dog, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human p38 MAPK Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CSBP antibody, CSBP1 antibody, CSBP2 antibody, CSPB1 antibody, EXIP antibody, Mxi2 antibody, PRKM14 antibody, PRKM15 antibody, RK antibody, SAPK2A antibody, p38 antibody, p38ALPHA antibody, MAPK14 antibody, mitogen-activated protein kinase 14 antibody, Csaids binding protein antibody, MAP kinase Mxi2 antibody, cytokine suppressive anti-inflammatory drug-binding protein antibody, mitogen-activated protein kinase p38 alpha antibody, p38 mitogen activated protein kinase antibody, MAX-interacting protein 2 antibody, CSAID-binding protein antibody, p38 MAP kinase antibody, cytokine suppressive anti-inflammatory drug binding protein antibody, stress-activated protein kinase 2A antibody, MAP kinase p38 alpha antibody, MAP kinase 14 antibody, p38alpha Exip antibody

Cellular Localization

Cytoplasm (By similarity) , Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX110720_40100_20180316_WB_KO_watermark_19011507_304.jpg

GTX110720 WB Image

KO/KD

Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX110720_40100_20141128_IHC_19011507_209.jpg

GTX110720 IHC-P Image

p38 antibody [N1C3-2] detects p38 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
p38 antibody [N1C3-2] (GTX110720) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110720_40100_20161208_WB_19011507_417.jpg

GTX110720 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX110720_40100_20151029_IFA_19011507_192.jpg

GTX110720 ICC/IF Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX110720_40100_20160201_IHC-P_R_19011507_903.jpg

GTX110720 IHC-P Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

p38 MAPK antibody [N1C3-2] Cat No. GTX110720

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Lane Description : Lane 1: PANC1, Lane 2: AsPC1, Lane 3: BxPC3
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf