p38 MAPK antibody

Cat No. GTX27952

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP, IHC
Reactivity	Human, Mouse, Rat

See all p38 MAPK products

Datasheet
Reference

APPLICATION

Application Note

For IHC: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

41 kDa. ( Note )

Positive Control

HeLa, HL-60, and NIH/3T3

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide (Mouse) identical to the corresponding human sequence.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CSAID Binding Protein 1 CSBP 1 Cytokine Supressive Anti-Inflamatory Drug Binding Protein 1 MAPK 14 Mitogen Activated Protein Kinase 14 SAPK 2A Stress Activated Protein Kinase 2A

Cellular Localization

Cytoplasm (By similarity) , Nucleus

Background

MAP kinases consist of several subgroups, including the ERK, JNK, and p38 kinases. The members are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides (1). These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis (1,2). The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation (3). The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif (4,5). While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif (6,7).