p38 MAPK (phospho Thr180 / Tyr182) antibody [HL2742]

Untreated (–) and treated (+) mouse tissue extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK (phospho Thr180 / Tyr182) antibody [HL2742] (GTX639571) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK (phospho Thr180 / Tyr182) antibody [HL2742] (GTX639571) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant (MAPK14 peptide, MAPK14 (phospho Thr180) peptide, MAPK14 (phospho Tyr182) peptide, and MAPK14 (phospho Thr180/Tyr182) peptide) (15.63 ng/mL). Coated protein was probed with p38 MAPK (phospho Thr180 / Tyr182) antibody [HL2742] (GTX639571) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.