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p38 MAPK (phospho Thr180/Tyr182) antibody

Orthogonal
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX24822
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX24822
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX24822
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24822
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX24822
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24822
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX24822

Cat. No. GTX24822

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all p38 MAPK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1 μg/ml
IHC-P 1:10-1:100
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

Reacts with p38 alpha, beta and gamma.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p38 that contains threonine 180 and tyrosine 182. This region is conserved among many species including mouse, rat, dog, monkey and carp.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380441

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX24822

GTX24822 IHC-P Image

IHC-P analysis of human brain tissue using GTX24822 p38 MAPK (phospho Thr180/Tyr182) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX24822

GTX24822 IHC-P Image

IHC-P analysis of rat heart tissue using GTX24822 p38 MAPK (phospho Thr180/Tyr182) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX24822

GTX24822 WB Image

Orthogonal

WB analysis of 20 μg of HeLa (Lane 1) exposed for 40 minutes with UV (lane2), A431 (Lane 3) exposed for 40 minutes with UV (lane4), COLO 205 (Lane 5) exposed for 40 minutes with UV (Lane 6), A549 (Lane 7) exposed for 40 minutes with UV (Lane 8) cell lysate using GTX24822 p38 MAPK (phospho Thr180/Tyr182) antibody.
Dilution : 1:1000

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24822

GTX24822 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX24822 p38 MAPK (phospho Thr180/Tyr182) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA incubated for 3 hours at room temperature

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX24822

GTX24822 IHC-P Image

IHC-P analysis of human heart tissue using GTX24822 p38 MAPK (phospho Thr180/Tyr182) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24822

GTX24822 ICC/IF Image

Orthogonal

ICC/IF analysis of RAW cells were untreated (-) or treated (+) with Anisomycin using GTX24822 p38 MAPK (phospho Thr180/Tyr182) antibody.
Green : Actin
Red : p38 MAPK (phospho Thr180/Tyr182) or JNK (phospho Tyr183/Tyr185)
Yellow : Composite

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX24822

GTX24822 WB Image

Orthogonal

WB (peptide competition) analysis of HEK 293 cells treated with UV irradiation using GTX24822 p38 MAPK (phospho Thr180/Tyr182) antibody prior incubated with no peptide (Lane 2), the non-phosphorylated peptide corresponding to the phosphopeptide (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4-5), and the phosphopeptide derived from the corresponding region of JNK1 and 2 (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

Application Reference

Yan Sun et al. Immun Inflamm Dis 2022; 10 (7) : e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Kuo-Hao Ho et al. Neurotherapeutics 2021; 18 (2) : 1371-1392 Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling.
Application : WB
Reactivity : Human
Huang C et al. BMC Complement Med Ther 2020; 20 (1) : 68 Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and -independent apoptosis via Smad or Akt signaling pathways in HOS cells.
Yang CH et al. Neurochem Res 2019; 44 (4) : 796-810 Long-term Therapeutic Effects of Extracorporeal Shock Wave-Assisted Melatonin Therapy on Mononeuropathic Pain in Rats.
Application : IHC-P
Reactivity : Rat
Ghasemi A et al. J Cell Biochem 2017; (Epub) Leptin Induces Matrix Metalloproteinase 7 Expression To Promote Ovarian Cancer Cell Invasion By Activating ERK And JNK Pathways.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399