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p38 MAPK (phospho Thr180/Tyr182) antibody

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunohistochemistry (IHC). GTX48614
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614

Cat. No. GTX48614

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC

Reactivity

 Human, Mouse
Package
100 μl ($499)
See all p38 MAPK products

APPLICATION

Application Note

We recommend the following starting dilutions:
For WB: Use at a dilution of 1:1000.
For IHC: Use a dilution of 1:250.
Optimal dilutions/concentrations should be determined by the end user.

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_10617651

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen activated protein kinase 14 , CRK1 , CSBP , CSPB1 , Csbp1 , Csbp2 , Exip , Hog , Mxi2 , Prkm14 , Prkm15 , RK , Sapk2A , p38 , p38Hog , p38alpha

Background

mitogen-activated protein kinase; involved in intracellular signalling, inhibition of apoptosis and gene activation [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunohistochemistry (IHC). GTX48614

GTX48614 IHC Image

Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red using p38 MAPK (phospho Thr180/Tyr182) antibody (GTX48614)

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614

GTX48614 WB Image

Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182 using p38 MAPK (phospho Thr180/Tyr182) antibody (GTX48614)

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614

GTX48614 WB Image

Western Blot: 38MAPK Phosphospecific Antibody [GTX48614] - Western blot of C-6 glioma cell lysates that had been incubated in the absence (Control) or presence of anisomycin showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.

REFERENCE

Application Reference

Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
Application : WB
Reactivity : Human
Chi-Cheng Lu et al. Exp Ther Med 2021; 22 (2) : 822 Dracorhodin perchlorate enhances wound healing via β-catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4) : 1271-1281
Chen CF et al. Oncol Lett 2018; 16 (5) : 6323-6330
Lin JT et al. Journal of Functional Foods 2018; 47 : 343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
Application : WB
Reactivity : Human
Chi-Cheng Lu et al. Exp Ther Med 2021; 22 (2):822 Dracorhodin perchlorate enhances wound healing via β-catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes.
Application : WB
Reactivity : Human
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Chen CF et al. Oncol Lett 2018; 16 (5):6323-6330
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Cho EC et al. Mediators Inflamm 2015; 2015:287345 RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.
Application : WB
Reactivity : Human
Er-Chieh Cho et al. Mediators of Inflammation 2015; RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress
Application : WB
Reactivity : Human
Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Application : WB
Reactivity : Mouse
Sinanoglu O et al. Urology 2012; 80 (6):1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.
Application : WB
Reactivity : Mouse
SDS
Glycerol.pdf
Package List Price ($)
$ 499