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p38 MAPK (phospho Thr180/Tyr182) antibody

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunohistochemistry (IHC). GTX48614
Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614

Cat No. GTX48614

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

We recommend the following starting dilutions:
For WB: Use at a dilution of 1:1000.
For IHC: Use a dilution of 1:250.
Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

P38 is a reactivating kinase that is a member of the MAP kinase family. It is activated in response to inflammatory cytokines, endotoxins, and osmotic stress. It is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002).

Database

Research Area

DATA IMAGES

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614

GTX48614 WB Image

Western Blot: 38MAPK Phosphospecific Antibody [GTX48614] - Western blot of C-6 glioma cell lysates that had been incubated in the absence (Control) or presence of anisomycin showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Immunohistochemistry (IHC). GTX48614

GTX48614 IHC Image

Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red using p38 MAPK (phospho Thr180/Tyr182) antibody (GTX48614)

Anti-p38 MAPK (phospho Thr180/Tyr182) antibody used in Western Blot (WB). GTX48614

GTX48614 WB Image

Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182 using p38 MAPK (phospho Thr180/Tyr182) antibody (GTX48614)

REFERENCE

REVIEW

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SDS
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