GeneTex
  • Country / Location Selection

United States (US)

p38 MAPK (phospho Thr180) antibody

Anti-p38 MAPK (phospho Thr180) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32319
Anti-p38 MAPK (phospho Thr180) antibody used in Western Blot (WB). GTX32319

Cat No. GTX32319

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of p38 MAPK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p38 MAPK (phospho Thr180) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32319

GTX32319 ICC/IF Image

ICC/IF analysis of formalin-fixed HepG2 cells using GTX32319 p38 MAPK (phospho Thr180) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-p38 MAPK (phospho Thr180) antibody used in Western Blot (WB). GTX32319

GTX32319 WB Image

WB analysis of PMA-treated HepG2 (A), NIH3T3 (B), PC12 (C) whole cell lysates using GTX32319 p38 MAPK (phospho Thr180) antibody.

REFERENCE

There are currently no references for p38 MAPK (phospho Thr180) antibody (GTX32319). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p38 MAPK (phospho Thr180) antibody (GTX32319). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319