GeneTex
United States (US)

p38 MAPK (phospho Thr180) antibody

GTX32319_20151013_WB_18121410_611.jpg
GTX32319_20151013_ICCIF_18121410_685.jpg

Cat No. GTX32319

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

41kDa kDa. ( Note )

Specificity/Sensitivity

Recognizes endogenous levels of p38 (pT180) protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p38 (pT180). The exact sequence is proprietary.

Purification

The antibody was purified by immunogen affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

RK Antibody , MAPK14 Antibody , CSBP Antibody , mitogen-activated protein kinase 14 Antibody , Mxi2 Antibody , SAPK2A Antibody , PRKM14 Antibody , CSBP1 Antibody , CSPB1 Antibody , p38 Antibody , PRKM15 Antibody , CSBP2 Antibody , p38ALPHA Antibody , EXIP

Cellular Localization

Cytoplasm (By similarity) , Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX32319_20151013_WB_18121410_611.jpg

GTX32319 WB Image

Western blot analysis of p38 (pT180) expression in HepG2 PMA-treated (A); NIH3T3 (B); PC12 (C) whole cell lysates.

GTX32319_20151013_ICCIF_18121410_685.jpg

GTX32319 ICC/IF Image

Immunofluorescent analysis of p38 (pT180) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).