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p38 MAPK (phospho Tyr182) antibody

Anti-p38 MAPK (phospho Tyr182) antibody used in Western Blot (WB). GTX50281
Anti-p38 MAPK (phospho Tyr182) antibody used in Western Blot (WB). GTX50281
Anti-p38 MAPK (phospho Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX50281

Cat No. GTX50281

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 182 (T-G-Y(p)-V-A) derived from human p38 MAPK.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p38 MAPK (phospho Tyr182) antibody used in Western Blot (WB). GTX50281

GTX50281 WB Image

WB analysis of extracts from HeLa cells treated with TNFa or calf intestinal phosphatase (CIP) using GTX50281 p38 MAPK (phospho Tyr182) antibody.

Anti-p38 MAPK (phospho Tyr182) antibody used in Western Blot (WB). GTX50281

GTX50281 WB Image

WB analysis of extracts from HeLa cells untreated or treated with UV for the indicated times using GTX50281 p38 MAPK (phospho Tyr182) antibody.

Anti-p38 MAPK (phospho Tyr182) antibody used in IHC (Paraffin sections) (IHC-P). GTX50281

GTX50281 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50281 p38 MAPK (phospho Tyr182) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf