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p38 gamma antibody [10E1]

Anti-p38 gamma antibody [10E1] used in Western Blot (WB). GTX84148
Anti-p38 gamma antibody [10E1] used in Flow cytometry (FACS). GTX84148
Anti-p38 gamma antibody [10E1] used in IHC (Paraffin sections) (IHC-P). GTX84148
Anti-p38 gamma antibody [10E1] used in IHC (Paraffin sections) (IHC-P). GTX84148

Cat. No. GTX84148

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10E1

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all p38 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human MAPK12 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 12 , ERK-6 , ERK3 , ERK6 , MAPK 12 , P38GAMMA , PRKM12 , SAPK-3 , SAPK3

Cellular Localization

Cytoplasm,Nucleus,Mitochondrion

Background

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.

Database

Research Area

DATA IMAGES

Anti-p38 gamma antibody [10E1] used in Western Blot (WB). GTX84148

GTX84148 WB Image

WB analysis of HEK293T cells transfected with p38 gamma plasmid (Right) or empty vector (Left) for 48 hrs using GTX84148 p38 gamma antibody [10E1].
Loading : 5 ug per lane

Anti-p38 gamma antibody [10E1] used in Flow cytometry (FACS). GTX84148

GTX84148 FACS Image

FACS analysis of HEK293T cells transfected with either p38 gamma plasmid(Red) or empty vector control plasmid(Blue) using GTX84148 p38 gamma antibody [10E1].

Anti-p38 gamma antibody [10E1] used in IHC (Paraffin sections) (IHC-P). GTX84148

GTX84148 IHC-P Image

IHC-P analysis of human breast tissue using GTX84148 p38 gamma antibody [10E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-p38 gamma antibody [10E1] used in IHC (Paraffin sections) (IHC-P). GTX84148

GTX84148 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84148 p38 gamma antibody [10E1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519