GeneTex
United States (US)

p40 antibody [N3C3]

GTX106557_IHC_19011506_434.jpg
GTX106557_40051_20150420_IP_19011506_395.jpg
GTX106557_WB_19011506_327.jpg
GTX106557_IFA_19011506_514.jpg

Cat No. GTX106557

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686P1077 antibody, RAB9P40 antibody, bA65N13.1 antibody, p40 antibody, RABEPK antibody, rab9 effector protein with kelch motifs antibody, 40 kDa Rab9 effector protein antibody, Rab9 effector p40 antibody

Cellular Localization

Cytoplasm , Endosome membrane

Background

Rab9 effector required for endosome to trans-Golgi network (TGN) transport.

Database

DATA IMAGES
GTX106557_IHC_19011506_434.jpg

GTX106557 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using p40 (GTX106557) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106557_40051_20150420_IP_19011506_395.jpg

GTX106557 IP Image

Immunoprecipitation of p40 protein from A431 whole cell extracts using 5 μg of p40 antibody [N3C3] (GTX106557).
Western blot analysis was performed using p40 antibody [N3C3] (GTX106557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106557_WB_19011506_327.jpg

GTX106557 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX106557 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106557_IFA_19011506_514.jpg

GTX106557 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using p40 (GTX106557) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169