p40 antibody [N3C3]

Cat. No. GTX106557

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all p40 products

Datasheet
Document

View product citations for antibody GTX106557 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951585

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rab9 effector protein with kelch motifs , RAB9P40 , bA65N13.1 , p40

Cellular Localization

Cytoplasm , Endosome membrane

Background

Rab9 effector required for endosome to trans-Golgi network (TGN) transport.

Database

Gene ID: 10244 RABEPK
UniProt: Q7Z6M1 RABEPK

Research Area

DATA IMAGES

GTX106557 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using p40 (GTX106557) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106557 IP Image

Immunoprecipitation of p40 protein from A431 whole cell extracts using 5 μg of p40 antibody [N3C3] (GTX106557).
Western blot analysis was performed using p40 antibody [N3C3] (GTX106557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106557 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX106557 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106557 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using p40 (GTX106557) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty