APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1:100-1:1900 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Sheep, Bovine, Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding mutant R175H of human p53. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909974
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
![Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395](/templates/default/js/lightbox/images/loading.gif "Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395")
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GTX636395 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636395 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636395 ICC/IF Image
p53 (R175H Mutant) antibody [HL1129] detects p53 (R175H Mutant) protein at nucleus by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 (R175H Mutant) stained by p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:1900.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636395 IHC-P Image
p53 (R175H Mutant) antibody [HL1129] detects p53 (R175H Mutant) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded AU565 Xenograft.p53 (R175H Mutant) stained by p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for p53 (R175H Mutant) antibody [HL1129] (GTX636395). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p53 (R175H Mutant) antibody [HL1129] (GTX636395). Be the first to share your experience with this product.
![Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395](/upload/website/prouct_img/small/GTX636395/GTX636395_45271_20231229_WB_B_24012217_576.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395")
![Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395](/upload/website/prouct_img/small/GTX636395/GTX636395_45131_20230811_WB_23102401_392.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395")
![Anti-p53 (R175H Mutant) antibody [HL1129] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636395](/upload/website/prouct_img/small/GTX636395/GTX636395_T-44403_20210924_ICC_IF_w_23061202_723.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636395")
![Anti-p53 (R175H Mutant) antibody [HL1129] used in IHC (Paraffin sections) (IHC-P). GTX636395](/upload/website/prouct_img/small/GTX636395/GTX636395_T-44403_20221111_IHC-P_22111518_581.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in IHC (Paraffin sections) (IHC-P). GTX636395")
![Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395](/upload/website/prouct_img/normal/GTX636395/GTX636395_45271_20231229_WB_B_24012217_576.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395")
![Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395](/upload/website/prouct_img/normal/GTX636395/GTX636395_45131_20230811_WB_23102401_392.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395")
![Anti-p53 (R175H Mutant) antibody [HL1129] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636395](/upload/website/prouct_img/normal/GTX636395/GTX636395_T-44403_20210924_ICC_IF_w_23061202_723.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636395")
![Anti-p53 (R175H Mutant) antibody [HL1129] used in IHC (Paraffin sections) (IHC-P). GTX636395](/upload/website/prouct_img/normal/GTX636395/GTX636395_T-44403_20221111_IHC-P_22111518_581.webp "Anti-p53 (R175H Mutant) antibody [HL1129] used in IHC (Paraffin sections) (IHC-P). GTX636395")