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p53 (R175H Mutant) antibody [HL1129]

Anti-p53 (R175H Mutant) antibody [HL1129] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636395
Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395
Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395

Cat No. GTX636395

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1129

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1900
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human p53 (R175H Mutant). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909974

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 (R175H Mutant) antibody [HL1129] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636395

GTX636395 ICC/IF Image

p53 (R175H Mutant) antibody [HL1129] detects p53 (R175H Mutant) protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 (R175H Mutant) stained by p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:1900.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395

GTX636395 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 (R175H Mutant) antibody [HL1129] used in Western Blot (WB). GTX636395

GTX636395 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

There are currently no references for p53 (R175H Mutant) antibody [HL1129] (GTX636395). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p53 (R175H Mutant) antibody [HL1129] (GTX636395). Be the first to share your experience with this product.
Package List Price ($)
$ 369
$ 169
Custom Conjugations