For WB: Use at a dilution of 1:1,000-1:5,000. Detects GST-p53 acetylated by recombinant p300, HAT domain in an in vitro acetyl transferase assay. Optimal dilutions/concentrations should be determined by the end user.
Detects GST-p53 acetylated by recombinant p300, HAT domain in an in vitro acetyl transferase assay.
Whole antiserum containing 0.05% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Synthetic peptide - amino acids 367-379 of human p53 (SHLKSK[AcK]GQSTSR)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Tumor Protein P53,Bcc7,Lfs1,P53,Trp53,Tp53
p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas. Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene. The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression. p53's role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors.