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p53 (acetyl Lys373) antibody

Anti-p53 (acetyl Lys373) antibody used in Western Blot (WB). GTX30768

Cat No. GTX30768

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Dog, Monkey
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
IP Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

Specific for human p53 acetylated in vitro by recombinant p300, and for peptide containing acetyllysine 373 but not for the peptide containing acetyllysine 320. Modest crossreactivity for p53 acetylated in vitro by recombinant PCAF.

PROPERTIES

Form

Liquid

Buffer

Serum, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated, synthetic peptide corresponding to aa367-379 of human p53 (SHLKSK[AcK]GQSTSR, where AcK denotes acetyllysine). The specificity was demonstrated with synthetic acetylated peptides.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCC7 , LFS1 , P53 , TP53 , TRP53 , tumor protein p53 , p53

Cellular Localization

Cytoplasm,Nucleus,PML body,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 (acetyl Lys373) antibody used in Western Blot (WB). GTX30768

GTX30768 WB Image

WB analysis of in vitro acetyl transferase reaction products using GTX30768 p53 (acetyl Lys373) antibody.Arrow indicates acetylated GST-p53.
Lane 1 : GST-p53 alone
Lane 2 : GST-p53 in the presence of PCAF
Llane 3: GST-p53 in the presence of p300
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319