*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Specific for human p53 acetylated in vitro by recombinant p300, and for peptide containing acetyllysine 373 but not for the peptide containing acetyllysine 320. Modest crossreactivity for p53 acetylated in vitro by recombinant PCAF.
Serum, 0.05% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
KLH-conjugated, synthetic peptide corresponding to aa367-379 of human p53 (SHLKSK[AcK]GQSTSR, where AcK denotes acetyllysine). The specificity was demonstrated with synthetic acetylated peptides.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
BCC7 , LFS1 , P53 , TP53 , TRP53 , tumor protein p53 , p53
Cytoplasm,Nucleus,PML body,Endoplasmic reticulum,Mitochondrion matrix
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
GTX30768 WB Image
WB analysis of in vitro acetyl transferase reaction products using GTX30768 p53 (acetyl Lys373) antibody.Arrow indicates acetylated GST-p53.
Lane 1 : GST-p53 alone
Lane 2 : GST-p53 in the presence of PCAF
Llane 3: GST-p53 in the presence of p300
Dilution : 1:1000