GeneTex
United States (US)

p53 (acetyl Lys386) antibody

p53-acetyl-Lys386-antibody-GTX88009-IHC-1_18121411_817.jpg
p53-acetyl-Lys386-antibody-GTX88009-ICC_IF-1_18121411_599.jpg

Cat No. GTX88009

Host Rabbit
Clonality Polyclonal
Application ICC/IF, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Specificity/Sensitivity

p53 (Acetyl-Lys386) Antibody detects endogenous levels of total p53 protein only when acetylated at lysine386.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys386.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
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Database

Research Area

DATA IMAGES
p53-acetyl-Lys386-antibody-GTX88009-IHC-1_18121411_817.jpg

GTX88009 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.

p53-acetyl-Lys386-antibody-GTX88009-ICC_IF-1_18121411_599.jpg

GTX88009 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys386) Antibody. The picture on the right is treated with the synthesized peptide.