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p53 antibody [B20.1 (BP 53.122)]

Cat No. GTX28590

Host

Mouse

Clonality

Monoclonal

Clone Name

B20.1 (BP 53.122)

Isotype

IgG2a

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Hamster, Monkey
Package
500 μl ($289)

APPLICATION

Application Note

This antibody can be used on formalin-fixed, paraffin-embedded tissue sections at a dilution of 1:50-1:75 . Heat mediated antigen retrieval is strongly recommended for consistent and reproducible results. For western blotting use at 1:100-1:250.This antibody is not suitable for frozen tissue sections. The optimal conditions should be determined by the individual laboratory.

Calculated MW

44 kDa. ( Note )

Positive Control

Breast Tumor - 50% of breat tumors are p53 positive, especially those lacking estrogen and/or progesterone receptors and with high proliferation index.

Product Note

This antibody reacts with wild type as well as mutant type of the p53 protein. It recognizes an epitope in the N-terminus of the human p53 protein. This antibody recognizes cell nuclei in which the p53 protein are generally localized. This antibody does not react with normal fibroblasts.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

IgG fraction

Conjugation

Unconjugated

RRID

AB_1241148

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

REFERENCE

REVIEW

p53 antibody [B20.1 (BP 53.122)] Cat No. GTX28590

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 29045486
SDS
Sodium Azide.pdf
Package List Price ($)
$ 289