APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes defined epitope (aa 16-25) on human p53.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Bacterially expressed full-length wild-type p53
Purification
Purified by size-exclusion chromatography
Conjugation
RRID
AB_625505
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCC7 , LFS1 , P53 , TP53 , TRP53 , tumor protein p53 , p53
Cellular Localization
Cytoplasm,Nucleus,PML body,Endoplasmic reticulum,Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
|
GTX78241 IHC Image
Confocal microscopy of human HeLa cells using anti-p53 (BP53-12; FITC) (GTX78241). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.
|
REFERENCE
There are currently no references for p53 antibody [BP53-12] (FITC) (GTX78241). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p53 antibody [BP53-12] (FITC) (GTX78241). Be the first to share your experience with this product.