GeneTex
United States (US)

p53 antibody [BP53-12]

p53-antibody-BP53-12__GTX27757-1_18121411_382.jpg

Cat No. GTX27757

Host Mouse
Clonality Monoclonal
Clone Name BP53-12
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity Human, Primate
APPLICATION

Application Note

ELISA: Use at an assay dependant concentration. ICC: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with the defined epitope (aa 16-25) on human p53, and recognizes p53/T complex.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (wild type) (Human).

Purification

Purified by precipitation methods

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P53,Bcc7,Lfs1,P53,Trp53,Tp53

Cellular Localization

Cytoplasmic and Nuclear

Background

This antibody is suitable in the research of p53 tumor suppressor gene function, in the detection of p53 expression and/or mutations in clinical specimens. The antibody is applicable as a possible marker in differential diagnosis and prognosis of p53 positive versus p53 negative human tumors.

Database

Research Area

DATA IMAGES
p53-antibody-BP53-12__GTX27757-1_18121411_382.jpg

GTX27757 IHC Image

Confocal microscopy of human HeLa cells using anti-p53 (BP53-12; FITC) (GTX78241). The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.

Package List Price ($)
$ 319