p53 antibody immunoprecipitates p53 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of p53 protein by 2.5 μg of p53 antibody (GTX118730) 12% SDS-PAGE The immunoprecipitated p53 protein was detected by p53 antibody (GTX118730) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

p53 antibody [C2C3], C-term detects p53 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
p53 antibody [C2C3], C-term (GTX118730) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: HCT116 cells with mock treatment for 24hr
B: HCT116 cells with 30uM cisplatin treatment for 24hr
10% SDS PAGE
GTX118730 diluted at 1:1000