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p53 antibody [DO-7]

Anti-p53 antibody [DO-7] used in IHC (Paraffin sections) (IHC-P). GTX34938
Anti-p53 antibody [DO-7] used in Western Blot (WB). GTX34938

Cat. No. GTX34938

Host

Mouse

Clonality

Monoclonal

Clone Name

DO-7

Isotype

IgG2b

Application

WB, IHC-P, IHC-Fr, FACS, IP

Reactivity

Human, Bovine, Monkey
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 37-45) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human wild type p53 protein expressed in E. coli.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCC7 , LFS1 , P53 , TP53 , TRP53 , tumor protein p53 , p53

Cellular Localization

Cytoplasm,Nucleus,PML body,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [DO-7] used in IHC (Paraffin sections) (IHC-P). GTX34938

GTX34938 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34938 p53 antibody [DO-7].

Anti-p53 antibody [DO-7] used in Western Blot (WB). GTX34938

GTX34938 WB Image

WB analysis of HeLa cell lysate using GTX34938 p53 antibody [DO-7].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529