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p53 antibody [DO-7]

Anti-p53 antibody [DO-7] used in Western Blot (WB). GTX75255
Anti-p53 antibody [DO-7] used in IHC (Paraffin sections) (IHC-P). GTX75255

Cat No. GTX75255

Host

Mouse

Clonality

Monoclonal

Clone Name

DO-7

Isotype

IgG2b

Application

WB, IHC-P, IHC-Fr, FACS, IP

Reactivity

Human, Bovine
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/1,000
IHC-P 1/1,000
IHC-Fr Assay dependent
FACS 1/50-1/100
IP Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human p53.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tumor Protein P53 , Bcc7 , Lfs1 , P53 , Trp53 , Tp53

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [DO-7] used in Western Blot (WB). GTX75255

GTX75255 WB Image

WB analysis of HEK293 cell lysate using GTX75255 p53 antibody [DO-7].

Anti-p53 antibody [DO-7] used in IHC (Paraffin sections) (IHC-P). GTX75255

GTX75255 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX75255 p53 antibody [DO-7].

REFERENCE

There are currently no references for p53 antibody [DO-7] (GTX75255). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p53 antibody [DO-7] (GTX75255). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf