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p53 antibody [DO1]

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Immunoprecipitation (IP). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

Cat No. GTX70214

Host Mouse
Clonality Monoclonal
Clone Name DO1
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

For WB: Use at a dilution of 1:500-1:10000, For IP: Use at a dilution of 1:100-1:500 , For IHC-P: Use at a dilution of 1:1,000. Antigen retrieval can be done with EDTA at pH8.0 - Some customers have stated that the product works well without antigen retrieval. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HCT116 , 293T (0.5 μM doxorubicin treatment for 24 hr) , HCT116(30 μM cisplatin treatment for 24 hr)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human p53 expressed in E. coli.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P53,Bcc7,Lfs1,P53,Trp53,Tp53

Background

The p53 antibody, clone DO1, specifically recognizes an epitope at the N-terminal end of the human p53 protein between amino acids 20-25. p53 is a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. And p53, as a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15, a mutation which reduces the ability of p53 to arrest cell growth.

Database

Research Area

DATA IMAGES
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Immunoprecipitation (IP). GTX70214

GTX70214 IP Image

Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [DO1] (GTX70214).
Western blot analysis was performed using p53 antibody [D01] (GTX70214).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

p53 antibody [DO1] Cat No. GTX70214

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : OS-RC-2 cells
Amount used : 30μg
Blocking : 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Application Reference
Clone Reference
SDS
PBS.pdf
Package List Price ($)
$ 319